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Table 2 Sequence and product size of primers used for RT-qPCR

From: Dietary polyunsaturated fatty acids affect PPARγ promoter methylation status and regulate the PPARγ/COX2 pathway in some colorectal cancer cell lines

Gene

Primer position

Primer (5′ → 3′)

Product length (bp)

Ref.

GAPDH

Forward

Reverse

GGTCGGAGTCAACGGATTTGG

TGATGACAAGCTTCCCGTTCT

194

[54]

COX2

Forward

Reverse

CCGGGTACAATCGCACTTAT

GGCGCTCAGCCATACAG

103

[55]

PPARγ

Forward

Reverse

GTCACGGAACACGTGCAGC

CAGGAGCGGGTGAAGACTCA

72

[56]