Table 1 Sequence, annealing temperature, and product size of primers used for methylation-specific PCR (MSP) reactions
Region | Primer | Sequence | Position | Annealing T (°C) | Product size (bp) |
|---|---|---|---|---|---|
PPARγ M1F | 5′-GTGGGTTTTATTGTGCGC-3′ | -123 to + 49 | 58 | 171 | |
PPARγ M1R | 5′-AACCGAATCGAACCGAAC-3′ | ||||
M1 | PPARγ U1F | 5′-GTGGGTTTTATTGTGTGT-3′ | -123 to + 49 | 50 | 171 |
PPARγ U1R | 5′-AACCAAATCAAACCAAAC-3′ | ||||
PPARγ M2F | 5′-CGGGGGTATTTTTTTAAATTTC-3′ | -151 to -270 | 54 | 119 | |
PPARγ M2R | 5′-ACTCTCTACCCCGCGACA-3′ | ||||
M2 | PPARγ U2F | 5′-GTTGGGGGTATTTTTTTAAATTTT-3′ | -151 to -272 | 54 | 121 |
PPARγ U2R | 5′-CCACTCTCTACCCCACAACA-3′ | ||||
PPARγM3F | 5′-AAGACGGTTTGGTCGATC-3′ | -235 to -359 | 52 | 124 | |
PPARγ M3R | 5′-CGAAAAAAAATCCGAAATTTAA-3′ | ||||
M3 | PPARγ U3F | 5′-GGGAAGATGGTTTGGTTGATT-3′ | -235 to -362 | 55 | 127 |
PPARγ U3R | 5′-TTCCAAAAAAAAATCCAAAATTTAA-3′ | ||||
PPARγ M4F | 5′-GAGATTAGCGGTTTTTTGAAC-3′ | -616 to -746 | 52 | 130 | |
M4 | PPARγ M4R | 5′-AAACGTAAAACACGAAAAACA-3′ | |||
PPARγ U4F | 5′-TAGGAGATTAGTGGTTTTTTGAAT-3′ | -615 to -749 | 52 | 134 | |
PPARγ U4R | 5′-AAAAACATAAAACACAAAAAACAA-3′ |