Skip to main content
Fig. 2 | Genes & Nutrition

Fig. 2

From: Dietary polyunsaturated fatty acids affect PPARγ promoter methylation status and regulate the PPARγ/COX2 pathway in some colorectal cancer cell lines

Fig. 2

Analysis of methylation patterns in four regions of the PPARγ promoter (M1-M4) in various colorectal cancer (CRC) cell lines treated with BSA alone as a control, and in those treated with polyunsaturated fatty acids (PUFAs). (A) DNA methylation profiles of four regions within the PPARγ promoter were identified using the MSP method. Cell lines showing both methylated (●) and unmethylated (○) variants are referred to as hemimethylated, in which methylation occurs in only one of two strands of DNA within the promoter region. (B) Representation of MSP products from various regions of the PPARγ promoter in CRC cell lines using agarose gel electrophoresis. C; control, EPA; eicosapentaenoic acid, DHA; docosahexaenoic acid, LA; linoleic acid

Back to article page

Genes & Nutrition

ISSN: 1865-3499

Contact us