Fig. 7

Body weight, fat pad weight, and blood glucose levels in male WT, DKO (double knockout for Znt8KO and SstKO), Znt8KO and SstKO mice. A Growth curve. The CD-fed DKO mice had significantly lower weight than the WT control at 15 weeks of age (*, p < 0.05). All four genotypic mice gained significant weight after 15 weeks on HFD compared to the respective CD group (##, p < 0.01). In addition, among the HFD challenged groups, the SstKO mice were significantly heavier than WT at 12 and 18 weeks of age (◊, p < 0.05). B Fat pad wet weight (mg). The HFD-fed mice accumulated significantly more fat than the CD-fed counterparts in all genotypic groups (**, p < 0.01; ***, p < 0.001). In the CD-fed groups, Znt8KO mice had the least amount of fat than the other three genotypic mice (*, p < 0.05). C The ratio of fat pad weight to body weight. The HFD-fed mice had increased ratios of fat pad weight to body weight compared to the CD-fed counterparts in all genotypic groups (**, p < 0.01; ***, p < 0.001). In the CD-fed groups, Znt8KO mice had the lowest fat to weight ratio than the other three genotypic mice (*, p < 0.05). D Fasting and non-fasting blood glucose levels over the dietary challenge period. *, p < 0.05; **, p < 0.01; ***, p < 0.001. All data are presented as mean ± S.E., n = 7–10/group. Body weight, fat pad weight, and blood glucose data were analyzed by a one-way or two-way ANOVA test to compare the means of multiple groups when appropriate followed by a post-hoc test (Tukey’s test). CD, chow diet; HFD, high-fat diet