Fig. 6

Oral glucose tolerance test (OGTT) and intraperitoneal insulin tolerance test (ITT) in male Znt8KO and WT mice. A Blood glucose levels during OGTT. Znt8KO mice fed CD had a significant increase in the blood glucose level at 120 min post the glucose load, compared to the WT control in the same dietary group (**, p < 0.01). B Area Under the Curve (AUC) of blood glucose levels during OGTT. No significance was found between the genotypic groups fed either diet. Fifteen-weeks HFD challenge had a limited effect on the AUC of blood glucose levels of either the Znt8KO or WT mice during OGTT. C Plasma insulin levels during OGTT. Znt8KO mice fed CD had significantly lower plasma insulin levels at 0 min before oral glucose challenge and 15, 30, 60, and 120 min after the challenge than the WT control in the same dietary group. #, p < 0.05; ##, p < 0.01. D AUC of plasma insulin levels during OGTT. *, (p < 0.05) or **, (p < 0.01) denotes significant difference between Znt8KO and WT mice within the same dietary group. E Blood glucose levels during ITT. F AUC of blood glucose levels during ITT. No significance was found between the genotypic groups fed either diet. All data are presented as mean ± S.E., n = 8–10/group. Blood glucose and plasma insulin data were analyzed by a one-way or two-way ANOVA test to compare the means of multiple groups when appropriate followed by a post-hoc test (Tukey’s test). CD, chow diet; HFD, high-fat diet