Fig. 10

Plasma glucagon, Glp1, Pyy, and leptin levels during OGTT in male mice fed either the chow diet or high-fat diet. A Glucagon levels. The * denotes the significant difference between DKO and WT or Znt8KO in the CD group (*, p < 0.05; **, p < 0.01). The # implies the significant difference between the SstKO and WT or Znt8KO in the CD groups (p < 0.05). The ◊ denotes the significant difference between DKO and WT or Znt8KO in the HFD dietary group (p < 0.05). The □ shows the significant difference of SstKO between the CD and HDF groups (p < 0.05). B AUC of plasma glucagon levels. C Glp1 levels. The * denotes the significant difference between DKO and WT or Znt8KO in the CD group (**, p < 0.01). The # implies the significant difference between the CD-fed SstKO and the WT or Znt8KO in the same dietary group (#, p < 0.05; ###, p < 0.001). The ◊ denotes the significant difference between DKO and WT, SstKO, or Znt8KO in the HFD dietary group (◊, p < 0.05: ◊◊◊, p < 0.001). The □ shows the significant difference of DKO between the CD and HDF groups (□, p < 0.05; □□, p < 0.01). D AUC of plasma Glp. E Pyy levels. The * denotes the significant difference between DKO and WT or Znt8KO in the CD group (*, p < 0.05; **, p < 0.01). The # implies the significant difference between the CD-fed SstKO and the WT or Znt8KO in the same dietary group ((#, p < 0.05; ##, p < 0.01). The ◊ denotes the significant difference between DKO and WT, SstKO, or Znt8KO in the HFD dietary group (◊, p < 0.05). The ∆ implies the significant difference between the HFD-fed SstKO and the WT or Znt8KO mice in the same dietary group (p < 0.05). F AUC of plasma Pyy. G Leptin levels. The # implies the significant difference between the HFD-fed SstKO and the WT, DKO, or Znt8KO in the same dietary group (#, p < 0.05; ##, p < 0.01). The leptin levels were significantly increased at all examined timepoints during OGTT in all genotypic groups after 15-weeks of HFD challenge compared to the respective levels in the CD groups (◊◊, p < 0.01). H AUC of plasma leptin. Panels B, D, F, and H, the * denotes the significant difference between groups as indicated (*, p < 0.05; **, p < 0.01; ***, p < 0.001). The groups with different letters indicate the significant difference between the two diets within the same genotype. All data are presented as mean ± S.E., n = 6–8/group. Data was analyzed by a one-way or two-way ANOVA test to compare the means of multiple groups when appropriate followed by a post-hoc test (Tukey’s test). CD, chow diet; HFD, high-fat diet