Fig. 1

Immunofluorescence study of ZnT8, Sst, and ghrelin in the pylorus of the mouse stomach. ZnT8 (green, A & D) was co-stained with Sst (red, B) or ghrelin (red, E). ZnT8, Sst, or ghrelin was detected in the cytoplasm of endocrine cells. Solid arrows indicate the cells stained with ZnT8 (A & D). Open arrows indicate the cells stained either Sst (B) or ghrelin (E). Nuclei were stained with DAPI in blue (F). Scale bar = 25 µm. We detected that a fraction of Sst-expressing endocrine cells in the pylorus co-expressed with ZnT8 (asterisks) (C). However, we did not observe co-expression of ZnT8 with ghrelin in the ghrelin-expressing endocrine cells in the merged images (F). Staining was repeated 2–3 times in the pyloric tissues isolated from the wild type B6 mice. ZnT8, zinc transporter 8 or Sla30a8; Sst, somatostatin